THE RAJAGOPAL LAB

Our laboratory focuses on organ regeneration and the application of developmental biology to human disease.

We utilize the lung as a model system, in part due to the abundance of respiratory diseases of unknown causes and without cures.

Check out our most recent publication here

Basal-Clara

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foxi1gfp acetylated tubulin merged 25um

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ciliated epithelial cells

The ciliated epithelial cells generated in vitro from Cystic Fibrosis skin fibroblast-derived induced pluripotent stem cells (iPSCs) after mucociliary differentaition of airway progenitors on transwell. Red staining is acetylated tubulin indicative of ciliated cells. Blue is the DNA staining.

secretory Club epithelial cells

The secretory Club epithelial cells generated ex vivo from mouse embryonic stem cells (mESC) after subcutaneous engraftment of airway progenitors in an immunodeficient mouse. Red staining is NKX2.1, a specific transcription factor for airway epithelia, green staining is Clara cell secretory protein 1a1 (CCSP) indicative of Club cells. Blue is the DNA staining.

airway epithelium

The airway epithelium (including ciliated cells and secretory cells) generated in vitro from expanded patient primary tracheal airway stem cells. Green staining is acetylated tubulin indicative of ciliated cells, while red staining is Clara cell secretory protein 1a1 (CCSP) indicative of secretory cells.

Basal-Clara

Describe your image

foxi1gfp acetylated tubulin merged 25um

Describe your image

ciliated epithelial cells

The ciliated epithelial cells generated in vitro from Cystic Fibrosis skin fibroblast-derived induced pluripotent stem cells (iPSCs) after mucociliary differentaition of airway progenitors on transwell. Red staining is acetylated tubulin indicative of ciliated cells. Blue is the DNA staining.

secretory Club epithelial cells

The secretory Club epithelial cells generated ex vivo from mouse embryonic stem cells (mESC) after subcutaneous engraftment of airway progenitors in an immunodeficient mouse. Red staining is NKX2.1, a specific transcription factor for airway epithelia, green staining is Clara cell secretory protein 1a1 (CCSP) indicative of Club cells. Blue is the DNA staining.

airway epithelium

The airway epithelium (including ciliated cells and secretory cells) generated in vitro from expanded patient primary tracheal airway stem cells. Green staining is acetylated tubulin indicative of ciliated cells, while red staining is Clara cell secretory protein 1a1 (CCSP) indicative of secretory cells.