A mature airway epithelium derived from a single dedifferentiated secretory cell that has been expanded ex vivo as a stem cell and then subsequently differentiated on an air-liquid interface. Cilia are stained for acetylated tubulin (red) and secretory cells are stained with SCGB1A1 (green) and nuclei are blue (DAPI).

Sphere generated from the culture of single mature cell that go on to dedifferentiate into basal stem cells (Keratin-5 staining in green). Nuclei are blue based upon DAPI staining.

The airway epithelium (including ciliated cells and secretory cells) generated in vitro from expanded patient primary tracheal airway stem cells. Green staining is acetylated tubulin indicative of ciliated cells, while red staining is Clara cell secretory protein 1a1 (CCSP) indicative of secretory cells.